Table 6.

Penicillin and tetracycline antimicrobial susceptibility data and identified AMR determinants

		Tetracyclines				Penicillin G
		MIC (mg/L)		tetM Plasmid	S10	MIC (mg/L)	β-lactamase	penA allele	PonA	MtrR	PenB
	Sample ID	DOX	TET			PEN
1	KNY_NGAMR1	0.75	0.064	Absent	V57M	0.064	NP	Pattern XIV	–	–	A121G, G120D, −N122
2	KNY_NGAMR2	1.5	0.5	Absent	V57M	64	TEM1a	Pattern II	L421P	G45D, −A13	G120D
3	KNY_NGAMR3	6	16	Present	V57M	48	TEM239	Pattern IX	L421P	A39T	–
4	KNY_NGAMR4	16	12	Present	V57M	8	TEM1	Pattern XIX	–	A39T	A121S, N122K
5	KNY_NGAMR5	12	16	Present	V57M	3	TEM1	Pattern IX	L421P	H105Y	A121G, G120N, −N122
6	KNY_NGAMR6	0.75	0.75	Absent	V57M	0.094	NP	Pattern II	–	A39T	–
7	KNY_NGAMR7	12	16	Present	V57M	> 256	TEM239	Pattern XXII	L421P	–	A121S, N122K
8	KNY_NGAMR8	0.5	0.5	Absent	V57M	0.064	NP	Pattern XIV	L421P	A39T	A121G, G120D, −N122
9	KNY_NGAMR10	24	32	Present	V57M	> 256	TEM1	Pattern II	–	A39T	–
10	KNY_NGAMR11	12	12	Present	V57M	12	TEM1	Pattern XIV	L421P	A39T	A121G, G120D, −N122
11	KNY_NGAMR13	12	12	Present	V57M	8	TEM1	Pattern XIV	L421P	A39T	A121G, G120D, −N122
12	KNY_NGAMR14	8	24	Present	V57M	64	TEM1	Pattern II	–	A39T	A121S, N122K
13	KNY_NGAMR15	16	32	Present	V57M	48	TEM1	Pattern XIV	L421P	A39T	A121G, −N122
14	KNY_NGAMR16	8	4	Present	V57M	0.19	NP	Pattern XIV	–	A39T	A121G, −N122
15	KNY_NGAMR17	12	16	Present	V57M	0.38	NP	Pattern XXII	L421P	T86A, D79N, H105Y	–
16	KNY_NGAMR18	32	24	Present	V57M	0.5	TEM1	Pattern XIX	–	A39T	–
17	KNY_NGAMR19	96	64	Presentb	V57M	0.19	NP	Pattern XIV	L421P	A39T	–
18	KNY_NGAMR20	16	24	Present	V57M	12	TEM1	Pattern IX	L421P	A39T	A121G, G120D, −N122
19	KNY_NGAMR21	16	24	Present	V57M	32	TEM1	Pattern XIV	L421P	A39T	A121G, G120D, −N122
20	KNY_NGAMR22	12	32	Present	V57M	2	TEM1	Pattern XIX	–	A39T	–
21	KNY_NGAMR23	16	8	Present	V57M	12	TEM1	Pattern IX	L421P	A39T	A121G, G120D, −N122
22	KNY_NGAMR24	16	16	Present	V57M	2	TEM1	Pattern XIX	–	A39T	–
23	KNY_NGAMR26	24	32	Present	V57M	8	TEM239	Pattern XIV	–	A39T	A121G, −N122
24	KNY_NGAMR27	16	32	Present	V57M	1	TEM239	Pattern XIV	–	A39T	–
25	KNY_NGAMR28	24	32	Present	V57M	96	TEM1	Pattern II	–	A39T	A121G, −N122
26	KNY_NGAMR29	4	12	Present	V57M	0.094	NP	Pattern XIV	L421P	A39T	A121G, −N122
27	KNY_NGAMR30	12	12	Present	V57M	12	TEM1	pattern XIV	L421P	A39T	A121G, −N122
28	KNY_NGAMR31	24	48	Present	V57M	0.38	NP	Pattern II	–	A39T	A121G, G120D, −N122
29	KNY_NGAMR32	1	0.5	Absent	V57M	8	TEM1	Pattern XIV	L421P	A39T	A121G, G120D, −N122
30	KNY_NGAMR33	24	32	Present	V57M	3	NP	Pattern XIV	–	A39T	A121G, −N122
31	KNY_NGAMR35	24	48	Present	V57M	> 256	TEM1	Pattern II	–	A39T	–
32	KNY_NGAMR41	12	12	Present	V57M	32	TEM1	Pattern XIX	–	A39T	–
33	KNY_NGAMR42	12	24	Present	V57M	32	TEM1	Pattern XIV	–	T86A, D79N, H105Y	–
34	KNY_NGAMR50	32	16	Present	V57M	16	TEM1	Pattern II	–	A39T	A121G, G120D, −N122
35	KNY_NGAMR53	16	6	Present	V57M	6	TEM239	Pattern XIV	L421P	A39T	A121G, −N122
36	KNY_NGAMR54	8	2	Present	V57M	6	NP	Pattern XXII	–	A39T	–

DOX Doxycycline, TET Tetracycline, PEN Penicillin ^(a)β-lactamase encoding plasmid with an Asian plasmid backbone, ^(b)Dutch TetM determinant, NP Not present, “-” no mutation identified, (−N122)- deletion of N at position 122 of PenB, (−A13)- Adenine deletion in the 13 bp inverted repeat region between the − 10 and − 35 hexamers of the mtrR promoter

Pattern XXII; D346, F505L, A511V, A517G H542N, P553V, K556Q, I557V, I567V, N575, A576V

Pattern IX; D346, F505L, A511V, A517G, P552L

Pattern XIX; D346, F505L, A511V, A517G, H542N, I567V, N575, A576V

Pattern XIV; D346, F505L, A511V, A517G, H542N

Pattern II; D346, F505L, A511V, A517G