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Splicing assay of the c.1921G > A variant in TMPRSS15 gene by a minigene assay. Gel electrophoresis of the RT-PCR product of minigene transcripts in HEK293 cells. Lane 1: DL1000 Plus DNA Marker (Vazyme, China); lane 2: pSPL3 (263 bp); lane 3: E16-WT (404 bp); lane 4: c.1921G > A (263 bp). The two fragments were directly sequenced (right panel).
