FIGURE 5.

Monosaccharide, pH value and polysaccharide detection in C. albicans 24-h-old biofilms. (A) Benedict’s test of 1% sucrose TYE with S. mutans MVs containing reducing sugars (C. a supernatant was the supernatant of C. albicans biofilm culture with 1% sucrose TYE medium, C. a + MVs supernatant was the supernatant of C. albicans biofilm culture with 1% sucrose TYE culture and S. mutans MVs medium). (B) Seliwanoff’s test of 1% sucrose TYE with S. mutans MVs containing fructose. (C) pH measurements of the culture medium supernatants. (D) Polysaccharide detected by an anthrone-sulfuric acid colorimetric assay (The experiments were performed in three distinct replicates, and the data are presented as the means ± SD, ***P < 0.001 vs control group, using PBS as control group).