Figure 3.

M10 prevented the collapse of intestinal epithelial barrier during colitis. (A) Intestinal epithelial barrier analysis by Immunofluorescence staining assay. (a) Normal mice; (b) Model mice; (c) Myricetin-treated mice; (d) M10-treated mice by 50 mg/kg; (e) Mesalazine-treated mice; (f) Statistical analysis the average of fluorescence density in the intestinal epithelial barrier in each group, *p < 0.05, **p < 0.01 vs. model mice as well as Mesalazine-treated mice. (B) Transmission electron microscopy (TEM) analyzed the colonic epithelial villi in normal mice (a); Model mice (b); Myricetin-treated mice (c); M10-treated mice by 50 mg/kg (d); Mesalazine-treated mice (e). As compared to normal mice, model mice exhibited villous shedding and disordered arrangement. The villi of colonic epithelial cells in M10-treated mice arranged neatly without shedding.