FIGURE 2.
The ASPL-TFE3 type 1 fusion transcript is expressed in ASPS-1 cells. ASPL-TFE3 fusion transcripts were detected using a forward primer corresponding to nt 548 to 569 of ASPL (AAAGAAGTCCAAGTCGGGCCA) and a reverse primer corresponding to nt 972 to 993 in exon 4 of TFE3 (CGTTTGATGTTGGGCAGCTCA). Expected fragment sizes were 195 bp for ASPL-TFE3 type 1 and 300 bp for ASPL-TFE3 type 2.5 ASPS indicates alveolar soft part sarcoma; ASPL-TFE3, alveolar soft part locus-transcription factor E3.