Table 3.
Rat H-H/RA-RA lung and right ventricle common top scoring upstream regulators
| Upstream Regulator | Molecule Type | Rat HH/RARA Lung |
Rat RV HH/RARA |
||||||
|---|---|---|---|---|---|---|---|---|---|
| Expr Log Ratio | Adjusted P Value | Activation z-Score | Predicted Activation State | Expr Log Ratio | Adjusted P Value | Activation z-Score | Predicted Activation State | ||
| β-Estradiol | Chemical | 2.27E-26 | 0.572 | 2.85E-13 | 0.770 | ||||
| Dexamethasone | Chemical | 2.54E-17 | 1.114 | 3.45E-16 | 0.034 | ||||
| Lipopolysaccharide | Chemical | 1.72E-23 | −4.662 | Inhibited | 6.66E-20 | −2.132 | Inhibited | ||
| IFNG | Cytokine | 2.99E-12 | −5.049 | Inhibited | 4.29E-12 | −2.702 | Inhibited | ||
| IL10 | Cytokine | 2.17E-09 | −0.894 | 2.71E-07 | −1.056 | ||||
| IL1B | Cytokine | −0.536 | 1.02E-14 | −5.377 | Inhibited | −1.151 | 5.30E-19 | −0.712 | |
| IL6 | Cytokine | −2.614 | 1.91E-16 | −1.441 | 2.71E-07 | −0.593 | |||
| TNF | Cytokine | 7.82E-19 | −6.062 | Inhibited | 3.62E-23 | −2.826 | Inhibited | ||
| TGFB1 | Growth factor | 5.11E-14 | −3.031 | Inhibited | 2.84E-09 | −1.996 | |||
| ESR1 | Receptor | 2.24E-08 | 1.612 | 4.11E-05 | 0.406 | ||||
| ERBB2 | Kinase | 4.57E-09 | 2.520 | Activated | 2.97E-09 | −0.389 | |||
| KLF2 | Transcription regulator | −1.035 | 5.23E-10 | 1.249 | 1.39E-09 | −0.543 | |||
| NFKBIA | Transcription regulator | 2.99E-12 | 1.174 | 4.03E-08 | −1.442 | ||||
Expression (Expr) log ratios indicate genes that were included in the differentially regulated gene lists in the lung or right ventricle (RV) analyses (q-value < 0.05). Activation status (indicated by z-score), as predicted by Ingenuity Pathway Analysis (IPA) tool, indicates strength of activation (positive value) or inhibition (negative value) status. Z-scores > |2.0| are indicated as “Activated” (> +2.0) or “Inhibited” (< −2.0). Most notable is global downregulation of immune response gene expression in both lung and RV tissue. The P value represents the chance that the given differentially expressed gene list fits a predicted pattern expected for a transcriptional regulator, otherwise known as an enrichment estimation. Also displayed is IPA analysis of the pattern of up- and downregulated genes and predicted functional “status” of the upstream regulator. In most cases, the actual predicted gene’s expression is not a component of the uploaded differentially expressed gene list but is a predicted regulator from both of these considerations (enrichment and observed differentially expressed up- or downregulation). ESR1, estrogen receptor α, ERBB2, Erb-B2 receptor tyrosine kinase 2; HH, in utero and postnatal hypoxia; IFNG, interferon γ; IL6, interleukin 6; IL10, interleukin 10; IL1B, interleukin 1β; KLF2, Krüppel-like factor 2; NFKBIA, NF-κ-B inhibitor α; RARA, in utero and postnatal room air; TGFB1, transforming growth factor β1; TNF, tumor necrosis factor α.