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. 2020 Sep 11;11:1144. doi: 10.3389/fphys.2020.571813

Figure 2.

Figure 2

A337T and A337G strongly suppressed heteromeric Kv7.2 + 7.3 currents. (A) Currents produced by the indicated combinations of subunits; voltage protocol is shown as an inset. (B) Current-voltage plots show significant reductions by A337T and A337G compared to control at voltages of −20 and above (**p < 0.01, ***p < 0.001, Two-way ANOVA, Bonferroni test, n = 10–20, see Table 1). (C) Conductance-voltage plots of WT only and mutant-containing channels (n = 5–20, *p = 0.02, ***p < 0.001, Two-way ANOVA, Bonferroni test; see Table 1). (D,E) Single exponential fits for heteromeric channels showing the (D) activation time constant and (E) deactivation time constant (at −40 mV, *p < 0.05, Two-way ANOVA, Bonferroni test, n = 6–10). (F) Western blot analysis of cell lysates (left lanes) and isolated biotinylated membrane surface proteins (right lanes) from CHO cells expressing Kv7.2 + Kv7.3 co-transfected with A337 variants (n = 5). Upper, representative results; lower, quantification performed as for Figure 1. Images are of the entire immunoblots are shown as supplementary information (Supplementary Figure S3).