Figure 3.

A337T homomers are less able to co-immunoprecipitate calmodulin than WT Kv7.2 homomers. (A) Representative western blot analysis and quantification of calmodulin (CaM) co-immunoprecipitated using anti-Kv7.2 antibodies and transfected human embryonic kidney (HEK) cells expressing WT Kv7.2, G279S, or A337T. CaM co-immunoprecipitated is significantly reduced for A337T (^*p = 0.04, One-way ANOVA, Tukey test, n = 5) but not G279S. In plots, CaM immunoprecipitated is normalized to wild type (WT). (B) Representative western blot analysis and quantification of CaM co-immunoprecipitated from cells expressing WT Kv7.2 + Kv7.3, alone or in a 1:1:2 ratio with G279S or A337T, show no differences between groups (n = 4). These cropped blot images are from full-length gels shown as supplementary information (Supplementary Figure S6).