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. 2020 Sep 11;11:1144. doi: 10.3389/fphys.2020.571813

Figure 4.

Figure 4

A337T does not disrupt localization of Kv7.2 or Kv7.3 subunits at the AIS of hippocampal neurons. (A) Representative image of cultured rat hippocampal neurons electroporated with WT Kv7.2 + Kv7.3 cDNAs at E18 and imaged after 7 DIV. Neurons were labeled with DAPI, anti-AnkG, anti-Kv7.2, and anti-Kv7.3. Merged (color) and greyscale images show Kv7.2 + Kv7.3 localized at the AIS (scale, for all images: 10 μm). (B) Intensity profile quantifications of AnkG, Kv7.2, and Kv7.3 antibody labeling along the AIS. (C–H) Representative images of hippocampal neurons electroporated with pore mutations G279S, A294V, and T274M and their respective intensity profile quantifications. Expression of these variants prevents concentration of Kv7.2 and Kv7.3 at the AIS. (I,J) Representative image of A337T and its intensity profile quantification show a normal pattern of Kv7.2 and Kv7.3 localization at the AIS (histograms: n = 69–104 neurons).