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. 2020 Sep 11;11:1144. doi: 10.3389/fphys.2020.571813

Table 1.

Current density and activation gating parameters of wild type or variant-containing Kv7.2 and Kv7.2/7.3 channels.

Transfection n Current density pA/pF, +40 mV p V1/2 (mV) p k, fold (mV/e) p
Kv7.2 13 89.6 ± 9.6 −21.7 ± 1.0 11.7 ± 0.5
A337G 6 6.5 ± 1.1 <0.0011 n/a n/a
A337T 10 4.9 ± 1.6 <0.0011 n/a n/a
Kv7.2 + A337G 11 20.2 ± 3.6 <0.0011 −24.1 ± 1.2 12.1 ± 1.9 NS
Kv7.2 + A337T 15 55.3 ± 11.9 0.0011 −23.5 ± 0.8 12.7 ± 1.0 NS
Kv7.2 + Kv7.3 20 416.0 ± 30.9 −21.5 ± 0.8 10.6 ± 0.4
Kv7.2 + Kv7.3 + A337G 9 146.4 ± 30.3 <0.0012 −21.7 ± 0.9 0.04 ± 1.0 NS
Kv7.2 + Kv7.3 + A337T 17 164.7 ± 18.6 <0.0012 −23.3 ± 1.2 11.7 ± 0.7 NS
Kv7.3 + A337G 6 87.8 ± 28.7 <0.0012 −23.7 ± 0.4 6.6 ± 0.5 <0.0012
Kv7.3 + A337T 12 88.5 ± 14.3 <0.0012 −18.0 ± 0.4 9.8 ± 0.5 <0.0012
Kv7.2 + PIP5K 10 238.8 ± 50.0 <0.0013 −39.6 ± 1.38 <0.0013 8.5 ± 0.6 <0.0013
A337G + PIP5K 10 48.4 ± 24.6 <0.0013
<0.0014
n/a n/a
A337T + PIP5K 9 47.5 ± 15.9 <0.0013
<0.0014
n/a n/a
Kv7.2 + A337G + PIP5K 9 199.0 ± 35.4 <0.0013
NS4
−34.8 ± 0.8 <0.0013
NS4
9.6 ± 0.9 NS
Kv7.2 + A337T + PIP5K 9 162.6 ± 47.9 <0.0013
NS4
−39.0 ± 0.5 <0.0013
NS4
10.0 ± 0.6 NS
Kv7.2 + Kv7.3 + PIP5K 8 643.0 ± 58.9 <0.0013 −33.5 ± 0.6 <0.0013 9.6 ± 0.7 NS
Kv7.2 + Kv7.3 + A337G + PIP5K 9 537.4 ± 46.9 <0.0013
NS5
−33.5 ± 0.5 <0.0013
NS5
9.2 ± 0.6 NS
Kv7.2 + Kv7.3 + A337T + PIP5K 9 725.4 ± 45.5 <0.0013
NS5
−33.0 ± 0.5 <0.0013
NS5
9.0 ± 0.6 NS
1

vs. WT Kv7.2 alone.

2

vs. WT Kv7.2 + Kv7.3 alone.

3

vs. same subunits, without PIP5K.

4

vs. Kv7.2 + PIP5K.

5

vs. Kv7.2 + Kv7.3 + PIP5K.