Table 1.

Current density and activation gating parameters of wild type or variant-containing Kv7.2 and Kv7.2/7.3 channels.

Transfection	n	Current density pA/pF, +40 mV	p	V1/2 (mV)	p	k, fold (mV/e)	p
Kv7.2	13	89.6 ± 9.6		−21.7 ± 1.0		11.7 ± 0.5
A337G	6	6.5 ± 1.1	<0.0011	n/a		n/a
A337T	10	4.9 ± 1.6	<0.0011	n/a		n/a
Kv7.2 + A337G	11	20.2 ± 3.6	<0.0011	−24.1 ± 1.2		12.1 ± 1.9	NS
Kv7.2 + A337T	15	55.3 ± 11.9	0.0011	−23.5 ± 0.8		12.7 ± 1.0	NS
Kv7.2 + Kv7.3	20	416.0 ± 30.9		−21.5 ± 0.8		10.6 ± 0.4
Kv7.2 + Kv7.3 + A337G	9	146.4 ± 30.3	<0.0012	−21.7 ± 0.9		0.04 ± 1.0	NS
Kv7.2 + Kv7.3 + A337T	17	164.7 ± 18.6	<0.0012	−23.3 ± 1.2		11.7 ± 0.7	NS
Kv7.3 + A337G	6	87.8 ± 28.7	<0.0012	−23.7 ± 0.4		6.6 ± 0.5	<0.0012
Kv7.3 + A337T	12	88.5 ± 14.3	<0.0012	−18.0 ± 0.4		9.8 ± 0.5	<0.0012
Kv7.2 + PIP5K	10	238.8 ± 50.0	<0.0013	−39.6 ± 1.38	<0.0013	8.5 ± 0.6	<0.0013
A337G + PIP5K	10	48.4 ± 24.6	<0.0013 <0.0014	n/a		n/a
A337T + PIP5K	9	47.5 ± 15.9	<0.0013 <0.0014	n/a		n/a
Kv7.2 + A337G + PIP5K	9	199.0 ± 35.4	<0.0013 NS4	−34.8 ± 0.8	<0.0013 NS4	9.6 ± 0.9	NS
Kv7.2 + A337T + PIP5K	9	162.6 ± 47.9	<0.0013 NS4	−39.0 ± 0.5	<0.0013 NS4	10.0 ± 0.6	NS
Kv7.2 + Kv7.3 + PIP5K	8	643.0 ± 58.9	<0.0013	−33.5 ± 0.6	<0.0013	9.6 ± 0.7	NS
Kv7.2 + Kv7.3 + A337G + PIP5K	9	537.4 ± 46.9	<0.0013 NS5	−33.5 ± 0.5	<0.0013 NS5	9.2 ± 0.6	NS
Kv7.2 + Kv7.3 + A337T + PIP5K	9	725.4 ± 45.5	<0.0013 NS5	−33.0 ± 0.5	<0.0013 NS5	9.0 ± 0.6	NS

¹vs. WT Kv7.2 alone.

²vs. WT Kv7.2 + Kv7.3 alone.

³vs. same subunits, without PIP5K.

⁴vs. Kv7.2 + PIP5K.

⁵vs. Kv7.2 + Kv7.3 + PIP5K.