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. 2020 Sep 11;8:567028. doi: 10.3389/fcell.2020.567028

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Copyright © 2020 Min, Long, Zhao, Zhen, Li, Li, Fan, Yu, Zhao and Qiao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Correlation of ACAT1 acetylation levels in PCOS granulosa cells with clinical outcomes. (A) Detection of ACAT1 acetylation in granulosa cells of 25 PCOS patients. Loading control was Coomassie blue staining. The acetylation value (grayscale ratio) was normalized with Coomassie blue staining. When the ratio of the acetylation level of ACAT1 Lys-174 to the expression level of ACAT1 protein was more than 2, it was classified as hyperacetylation subgroup, while the hypoacetylation subgroup was defined when the ratio was less than 2. (B,C) showed the correlation analyses of ACAT1 acetylation levels with the 2PN fertilization rate and rate of transferable embryos. R: Pearson correlation coefficient.