TABLE 2.

The clinical outcomes of PCOS women with different acetylation level of ACAT1.

	Hyperacetylation	Hypoacetylation	p-value
Number	15	10
No. of MII oocyte	13.6 ± 9.1	13.1 ± 6.9	0.88
No. of 2PN embryos	7.9 ± 5.7	8.9 ± 4.5	0.66
2PN fertilization rate	56.5%	69.3%	0.01
No. of 2PN cleavage embryos	7.9 ± 5.4	8.9 ± 4.3	0.63
Cleavage rate	56.2%	69.3%	0.01
No. of transferable embryos	4.7 ± 5.1	8.1 ± 4.2	0.09
Rate of transferable embryos	52.5%	91.7%	0.01
Clinical pregnancy rate	12.5% (2/15)	50% (5/10)	0.17

The granulosa cell samples of 25 PCOS patients were examined to analyze the correlation of the lysine acetylation of ACAT1 protein to the clinical outcomes. The acetylation value (grayscale ratio) was normalized with loading control, Coomassie blue staining. When the ratio of the acetylation level of ACAT1 Lys-174 to the expression level of ACAT1 protein was more than 2, it was classified as hyperacetylation subgroup, while the hypoacetylation subgroup was defined when the ratio was less than 2. The IVF outcome measure: 2PN fertilization rate was defined as the number of two pronuclear (2PN) embryos divided by the number of MII oocytes; cleavage rate was defined as the number of 2PN cleavage embryos divided by the number of MII oocytes; transferable embryos were defined as day-3 embryos that reached the 4-cell stage, and the rate of transferable embryos was defined as the number of transferable embryos divided by the number of 2PN embryos; clinical pregnancy was defined as a presence of intrauterine gestational sac observed on ultrasound after 30 days of embryo transfer. The bold p-value means the significant difference between two groups.