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. 2020 Sep 24;11:4828. doi: 10.1038/s41467-020-18580-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Mechanical compression using a microfluidic compression device. a Design and b graph of compression-induced nuclear deformation with respect to the pressure applied, represented as ratio of its initial uncompressed area. In inlet, graph representing step-wise increase of pressure used for the experiment. c Time-lapse images of perinuclear cGAS foci formation in control and ATR-depleted cells under compression. d Amount of pressure applied through the microfluidic pump during the time of NE rupture (formation of first foci of cGAS) (n = 19, 24, and 21 shCtrl, shATR1, and ShATR2, respectively; data pooled from N = 2 independent replicates). All graphs presented as mean ± SEM. p-values are calculated using two-way ANOVA test and Tukey’s multiple comparisons test for b and one-way ANOVA test with Dunnett’s multiple comparisons test for d (**** if P < 0.0001, *** if P < 0.001).