Figure 2.

P130Cas is required for caudal vein plexus (CVP) angiogenesis. (A,B) Maximum intensity projections of wildtype and bcar1^u7000 embryos at 30hpf (A) and 48hpf (B), with inset (blue/orange dashed boxes) showing the sprouting front. Insets are inverted for easier visualisation. Blue line indicates ventral extension, magenta circles endothelial cell gaps, orange arrowheads filopodia-like cell protrusions. (C,D) Quantification of total CVP area, maximal ventral extension, and total non-vascularised area (= ‘EC gaps’) show a significant reduction at both time points. All results displayed as box plots with centre line showing median and box dimensions indicating 25th and 75th quartile. Each data point represents an individual embryo, experimental n from ≥ 3 independent clutches, p values as shown. White boxes are WT, light grey boxes indicate bcar1^u7000. Scale bars as indicated.