Figure 3.

P130Cas deletion results in prolonged reduction of caudal vein plexus area. (A) Maximum intensity projections of the caudal vasculature of WT and bcar1^u7000 embryos at 72hpf, from the anal pore, as illustrated by orange box in cartoon of embryo at the top. (B–D) Quantification of the maximum ventral extension of the CVP to the dorsal aorta, as well as overall vascular CVP area confirm that significant reductions remain in mutant embryos at 3dpf (B) and 4dpf (C). At 5dpf, the caudal area is still smaller but the ventral extension no longer significantly different (D). All results displayed as box plots with centre line showing median and box dimensions indicating 25th and 75th quartile. Each data point represents an individual embryo, experimental n from ≥ 2 independent clutches, p values as shown. White boxes are wildtype, light grey boxes indicate bcar1^u7000. Scale bar as indicated.