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. 2020 Sep 24;10:15706. doi: 10.1038/s41598-020-72736-7

Table 1.

qPCR primer sequences indicated in 5´–3´ direction for human gene targets with gene symbols and alternative names used in the manuscript (fw as forward, rev as reverse).

Gene Gene symbol NCBI accession number Primer sequence
Alkaline phosphatase ALP NM_000478.6

fw ACTGCAGACATTCTCAAA

rev GAGTGAGTGAGTGAGCA

Cementum protein 1 CEMP1 NM_001048212.3

fw GCCAAGGGGACACAGAAGAT

rev GGTGCTGGAGGGCAGTATG

Matrix metallopeptidase 3 MMP-3 NM_002422.5

fw ACCCTTTTGATGGACCTGGA

rev GGCTGAGTGAAAGAGACCCA

Matrix metallopeptidase 9 MMP-9 NM_004994.3

fw ATTTCTGCCAGGACCGCTTC

rev TCATAGGTCACGTAGCCCACT

Bone gamma-carboxyglutamate protein

BGLAP

(alias OCN)

NM_199173.6

fw CAGGCGCTACCTGTATCA

rew CTGGAGTTTATTTGGGAG

Secreted phosphoprotein 1

SSP1

(alias OSP)

NM_001040058.2

fw TGATTTTCCCACGGACCTGC

rev TCGCTTTCCATGTGTGAGGT

TNF receptor superfamily member 11b

TNFRSF11B

(alias OPG)

NM_002546.4

fw GAAGGGCGCTACCTTGA

rev GCAAACTGTATTTCGCTC

TNF Superfamily Member 11

TNFSF11

(alias RANKL)

NM_003701.4

fw ATCACAGCACATCAGAGCAGA

rev TCACTTTATGGGAACCAGATGGG

RUNX family transcription factor 2 RUNX2 NM_001015051.3

fw CCCACGAATGCACTATCC

rev GGACATACCGAGGGACA

Ribosomal protein L22 RPL22 NM_000983.4

fw TGATTGCACCCACCCTGTAG

rev GGTTCCCAGCTTTTCCGTTC

TATA-box binding

protein

TBP NM_003194.5

fw CGGCTGTTTAACTTCGCTTCC

rev TGGGTTATCTTCACACGCCAAG

Vascular Endothelial Growth Factor A VEGFA NM_001171623.1

fw TCCAGGAGTACCCTGATGAGAT

rev TGGTGAGGTTTGATCCGCATA