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. 2020 Sep 24;10:15601. doi: 10.1038/s41598-020-71943-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of PI3K, p-PI3K, AKT, p-AKT protein expression by Western blotting. (A) Immunoblotting for the target molecule of PI3K, p-PI3K, AKT, and p-AKT showing the effects of SDAPR, SDAP1 and SDAP2 in GM treated HEK-293 cells; (B) expression of p-PI3K/PI3K was determined by western blotting; (C) expression of p-AKT/AKT was determined by western blotting. Data are means ± SD (n = 3). **P < 0.01 as compared to the Control group. ^##P < 0.01 as compared to the GM-treated group.