KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| AffiniPure Fab Fragment Donkey Anti-Rat IgG (H+L) | Jackson Immuno, West Grove, PA | Cat# 712-007-003; RRID: AB_2340634 |
| biotin mouse anti-HuC/D | Invitrogen, Carlsbad, CA | Cat# A21272; RRID: AB_2535822 |
| chicken anti-GFAP | Abcam, Cambridge, MA | Cat# ab4674; RRID: AB_304558 |
| chicken anti-GFAP | Aves, Tigard, OR | Cat# GFAP87867983; RRID: AB_2313547 |
| donkey anti-chicken Alexa 488 | Jackson Immuno, West Grove, PA | Cat# 703-545-155; RRID: AB_2340375 |
| donkey anti-guinea pig DyLight 405 | Jackson Immuno, West Grove, PA | Cat# 706-475-148; RRID: AB_2340470 |
| donkey anti-rabbit Alexa 594 | Jackson Immuno, West Grove, PA | Cat# 711-585-152; RRID: AB_2340621 |
| donkey anti-rat Alexa 647 | Jackson Immuno, West Grove, PA | Cat# 712-605-150; RRID: AB_2340693 |
| goat anti-chicken Alexa 488 | Invitrogen, Carlsbad, CA | Cat# A-11039; RRID:AB_2534096 |
| goat anti-chicken DyLight 405 | Jackson Immuno, West Grove, PA | Cat# 103-475-155; RRID: AB_2337389 |
| goat anti-rabbit Alexa 488 | Invitrogen, Carlsbad, CA | Cat# A-11034; RRID:AB_2576217 |
| goat anti-rat Alexa 594 | Jackson Immuno, West Grove, PA | Cat# 112-585-003; RRID: AB_2338372 |
| guinea pig anti-PGP9.5 | Neuromics, Edina, MN | Cat# GP14104; RRID: AB_2210625 |
| rabbit anti-CCR2 | Abcam, Cambridge, MA | Cat# ab216863; RRID:AB_2832204 |
| rabbit anti-M-CSF | Bioss, Woburn, MA | Cat# bs-4910R; RRID:AB_2832205 |
| rabbit anti-S100β | Abcam, Cambridge, MA | Cat# ab52642; RRID: AB_882426 |
| rat anti-CD68, clone FA-11 | AbD Serotec, Raleigh, NC | Cat# MCA1957; RRID: AB_322219 |
| rat anti-F4/80 | Abcam, Cambridge, MA | Cat# ab6640; RRID: AB_1140040 |
| rat anti-M-CSF, clone 131614 | Bio-Techne Ltd, Minneapolis, MN | Cat# MAB4161; RRID:AB_2276674 |
| rat anti-MHC class II | BioLegend, San Diego, CA | Cat# 107602; RRID: AB_313317 |
| rat anti-MHC class II | Novus, Centennial, CO | Cat# NBP2-21789; RRID:AB_2828034 |
| streptavidin Alexa 594 | Jackson Immuno, West Grove, PA | Cat# 016-580-084; RRID: AB_2337250 |
| streptavidin DyLight 405 | Jackson Immuno, West Grove, PA | Cat# 016-470-084; RRID: AB_2337248 |
| TruStain FcX PLUS (anti-mouse CD16/32) Antibody | BioLegend, San Diego, CA | Cat# 156604; RRID:AB_2783138 |
| Biological Samples | ||
| Human intestinal tissue | Michigan State University, East Lansing, MI | N/A |
| Human intestinal tissue | The Ohio State University, Columbus, OH | N/A |
| Human intestinal tissue | University of California, Los Angeles, CA | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| 43Gap26 | AnaSpec Inc. | AS-62644 |
| ACCUTASE | STEMCELL Technologies Inc, Vancouver, Canada | 07920 |
| Amphotericin B | Life Technologies | 15290018 |
| Anti-fibroblast microbeads, human | Miltenyi Biotec Inc., San Diego, CA | 130-050-601 |
| Bovine serum albumin | Millipore-Sigma | A2153 |
| Corning® Laminin, Mouse, 1 mg | Corning Inc., Corning, NY | 354232 |
| DAPI Fluoromount-G® | SouthernBiotech, Birmingham, AL | 0100-20 |
| Deoxyribonuclease I (DNase I) Type IV | Millipore-Sigma | D5025-15KU |
| Dextran Sodium Sulfate (DSS) | MP Biomedical, Solon, OH | 0216011080 |
| DNase | Millipore-Sigma | D4527 |
| Dulbecco’s modified Eagle’s medium (DMEM)-F12, HEPES, no phenol red | Life Technologies | 11039021 |
| Fetal bovine serum | Life Technologies | 26140-079 |
| Fetal bovine serum | Denville Scientific, Inc., Holliston, MA | FB5001 |
| gentleMACS C Tubes | Miltenyi Biotec Inc. | 130-093-237 |
| gentleMACS Dissociator | Miltenyi Biotec Inc | 130-093-235 |
| GIBCO G-5 Supplement (100X) | Thermo Fisher Scientific | 17503012 |
| GIBCO HBSS (10X), calcium, magnesium, no phenol red | ThermoFisher Scientific Inc., Waltham, MA | 14065056 |
| GIBCO N-2 Supplement (100X) | Thermo Fisher Scientific | 17502048 |
| HEPES | Millipore Sigma | H3375 |
| Isoflurane | Henry Shein, Dublin, OH | 029405 |
| Liberase TH Research Grade | Millipore-Sigma | 5401151001 |
| Liberase TM Research Grade | Millipore-Sigma | LIBTM-RO |
| Mouse NGF-β, 20 μg | Cell Guidance Systems Ltd, St. Louis, MO | GFM11-20 |
| Nonidet P 40 Substitute | Millipore Sigma | 74385 |
| Paraformaldehyde | Millipore Sigma | 158127 |
| Penicillin/ Streptomycin solution 100X | Millipore Sigma | P4333 |
| Picric acid | Millipore Sigma | P6744 |
| Poly-D-Lysine solution, 1.0 mg/mL | MilliporeSigma | A-003-E |
| Protease inhibitor cocktail | Millipore Sigma, Burlington, MA | P8340-5ML |
| Recombinant human IL-1β | InvivoGen | rcyec-hil1b |
| Recombinant human M-CSF | Novus | 216-MC |
| Recombinant mouse IL-1β | R&D Systems | 401-ML-005 |
| SIGMAFAST Protease Inhibitor Tablets | Millipore Sigma | S8820 |
| Tamoxifen citrate in the chow (400 mg/kg) | Envigo | TD.140849 |
| Tris Buffered Saline (TBS), 10X | Fisher BioReagents | BP2471-1 |
| Zombie NIR Fixable Viability Kit | BioLegend, San Diego, CA | 423106 |
| Critical Commercial Assays | ||
| A magnetic bead separation column | Miltenyi Biotec Inc., San Diego, CA | 130-042-201 |
| Amicon Ultra-2 Centrifugal Filter Unit | MilliporeSigma, Burlington, MA | UFC201024 |
| Bicinchoninic Acid (BCA) Kit for Protein Determination | Sigma-Aldrich Company Ltd., St. Louis, MO | BCA1 |
| Mouse 31-Plex Cytokine Array / Chemokine Array | Eve Technologies Corp., Calgary, AB, Canada | SKU: MD31 |
| PKC Kinase Activity Assay Kit | Abcam, Cambridge, MA | ab139437 |
| RayBio® Human M-CSF ELISA Kit | RayBiotech Life, Peachtree Corners, GA | ELH-MCSF-1 |
| SensoLyte ® 520 TACE (α - Secretase) Activity Assay Kit *Fluorimetric* | AnaSpec, Inc., Fremont, CA | AS-72085 |
| Deposited Data | ||
| Next-generation sequencing of distal colon glial cells with DNBS-induced inflammation and neurokinin-2 receptor antagonism utilizing RiboTag mice | Delvalle et al., 2018 | Database: GSE114780 |
| Contributors: Gulbransen B. D., Delvalle N.M., and Dharshika C. | ||
| Cytokine/ chemokine multiplex array raw data | This paper | Table S3 |
| Experimental Models: Organisms/Strains | ||
| B6.129S7-Gja1tm1Dlg/J | Jackson Laboratory, Bar Harbor, ME | RRID: IMSR_JAX:008039 |
| Tg(Sox10-icre/ERT2)93Vpa | Dr. Vassilis Pachnis, The Francis Crick Institute, London, England | MGI ID: 5910373 |
| Oligonucleotides | ||
| hCx26 GGCTGTCTGTTGTATTCATTGTGGTCATAGCACCTAACAACATTGTAGCCTCAATCGAGTGAGACAGACTAGAAGTTCCTAGTGATGGCTTATGATAGCA | This paper | NM_004004.5 |
| hCx30 AGGCACGAAACCACTCGCAAGTTCAGGCGAGGAGAGAAGAGGAATGATTTCAAAGACATAGAGGACATTAAAAAGCAGAAGGTTCGGATAGAGGGGTCGC | This paper | NM_006783.4 |
| hCx31 GGTGGACCTACCTGTTCAGCCTCATCTTCAAGCTCATCATTGAGTTCCTCTTCCTCTACCTGCTGCACACTCTCTGGCATGGCTTCAATATGCCGCGCCT | This paper | NM_001005752.1 |
| hCx43 GCGAACCTACATCATCAGTATCCTCTTCAAGTCTATCTTTGAGGTGGCCTTCTTGCTGATCCAGTGGTACATCTATGGATTCAGCTTGAGTGCTGTTTAC | This paper | NM_000165.3 |
| hCx45 TTGCTGGCAAGGACCGTGTTTGAGGTGGGTTTTCTGATAGGGCAGTATTTTCTGTATGGCTTCCAAGTCCACCCGTTTTATGTGTGCAGCAGACTTCCTT | This paper | NM_001080383.1 |
| hCx47 GGAATGGGGCTCTGGGTTCCTGCCTGTGGCCTGTCTGTCCTCCTCCCTAATTCAGACCCAGCCTCAAGAGGAAAGGGAGTAAAATAAAACTAACTTGTTT | This paper | NM_020435.2 |
| ADA1 GCAGGTGCACAGGGAAGTCATCCCTACACATACTGTCTATGCTCTTAACATTGAAAGGATCATCACGAAACTCTGGCATCCAAATCATGAAGAGCTGCAG | This paper | NM_053053.3 |
| CTNNB1 TCTTGCCCTTTGTCCCGCAAATCATGCACCTTTGCGTGAGCAGGGTGCCATTCCACGACTAGTTCAGTTGCTTGTTCGTGCACATCAGGATACCCAGCGC | This paper | NM_001098210.1 |
| NMNAT1 CCGAGAAGACTGAAGTGGTTCTCCTTGCTTGTGGTTCATTCAATCCCATCACCAACATGCACCTCAGGTTGTTTGAGCTGGCCAAGGACTACATGAATGG | This paper | NM_022787.3 |
| RBP1 TGATCATCCGCACGCTGAGCACTTTTAGGAACTACATCATGGACTTCCAGGTTGGGAAGGAGTTTGAGGAGGATCTGACAGGCATAGATGACCGCAAGTG | This paper | NM_002899.3 |
| Software and Algorithms | ||
| BD FACSDiva v8.0.1 | Becton, Dickinson and Company, Franklin Lakes, NJ | RRID:SCR_001456 |
| FCS Express 7 Research Edition (Win64) v7.01.0018. | De Novo Software, Pasadena, CA | RRID:SCR_016431 |
| Huygens Professional software v17.10 | Scientific Volume Imaging, the Netherlands, | http://svi.nl RRID:SCR_014237 |
| ImageJ and Fiji | National Institutes of Health, Bethesda, MD | RRID:SCR_003070, RRID:SCR_002285 |
| Imaris v9.1.2 | Oxford Instruments, Abingdon, United Kingdom | RRID:SCR_007370 |
| LabChart 7 | ADInstruments, Colorado Springs CO | RRID:SCR_001620 |
| MetaMorph 7.0 | Molecular Devices, LLC, San Jose, CA | RRID:SCR_002368 |
| Prism 7 | GraphPad Software, San Diego, CA | RRID:SCR_002798 |