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. Author manuscript; available in PMC: 2020 Sep 25.
Published in final edited form as: Nat Med. 2019 Apr 22;25(6):947–953. doi: 10.1038/s41591-019-0421-7

Extended Data Fig. 7 |. In vivo expansion and memory generation.

Extended Data Fig. 7 |

a, Flow cytometry analysis showing in vivo expansion of tEGFR+ CD19-BBz(86) CAR T cells in the peripheral blood of representative patients who achieved complete remission (patient BZ021) or had progressive disease (patient BZ013). b,c. qPCR showing in vivo CAR T cell expansion and persistence in the blood of patients who achieved complete or partial remission (patients BZ015, BZ016, BZ019, BZ020, BZ021, BZ024 and BZ025) (b) or had progressive disease (patients BZ017, BZ022 and BZ023) (c). d, Memory phenotype (CD45RA CCR7+) of in vivo-expanded tEGFR+CD3+ CD19-BBz(86) CAR T cells and tEGFRCD3+ normal T cells from six patients who had progressive disease (BZ013) or achieved partial remission (BZ014) or complete remission (BZ015, BZ019, BZ020 and BZ021). e, Percentage of CD45RA+ and CD45RA subpopulations of in vivo-expanded tEGFR+CD3+ CD19-BBz(86) CAR T cells and tEGFRCD3+ normal T cells from the six treated patients. P values were calculated from two-tailed Student’s t tests. Horizontal lines denote median values. f, Percentage of CD45RA+CCR7, CD45RA+CCR7+, CD45RACCR7 and CD45RACCR7+ subpopulations of in vivo-expanded tEGFR+CD3+ CD19-BBz(86) CAR T cells and tEGFRCD3+ normal T cells from the six treated patients. P values were calculated from two-tailed Student’s t tests. Horizontal lines denote median values. gj, Detection of tEGFR+CD3+ CD19-BBz(86) CAR T cells in the peripheral blood of patient BZ015 on day 317 after cell infusion. g, Of the tEGFR+CD3+ CAR T cells, 96.7% were CD8+. h, CD19+ B cells were still depleted. i, 45.8% of the long-term, persistent tEGFR+CD3+ CD19-BBz(86) CAR T cells were CD45RO+CCR7+ central memory T cells in the peripheral blood of patient BZ015 on day 317 after cell infusion, while only 6.25% of the tEGFRCD3+ normal T cells were CD45RO+CCR7+ central memory T cells. j, qPCR analyses in triplicate also showed long-term persistence of CD19-BBz(86) CAR T cells in peripheral blood.