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. 2020 Sep 24;6:33. doi: 10.1038/s41522-020-00143-x

Fig. 3. Poloxamer fixation enables concurrent analysis of microbes and tissues, as compared with Carnoy’s and Metharn fixation.

Fig. 3

IF of proteins coupled to bacterial FISH over multiple rounds of MxIF, with universal bacterial probe (Eub, white), nuclei (blue), GOB5 (green), lectin (WGA, red), and pan-cytokeratin (PCK, yellow). Selected individual channels (PCK, GOB5, Eub) to visualize epithelium, mucus, and bacteria, with the EUB probe having the same exposure times and gains to compare signal-to-noise in the two conditions. a–d Carnoy’s solution, e–h Methacarn, and i–l Poloxamer fixative were used for processing the same distal colonic tissue. A* ×10 gain was used to detect Eub probe in the Carnoy’s condition. White dotted lines represent epithelial borders. Scale bars = 50 µm.