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. 2020 Sep 24;11:4830. doi: 10.1038/s41467-020-18376-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Representative lipid-channel SRS images from the same set of M381 cells before (top) and after (bottom) detergent wash in control (CT), with SC or CAY treatment. Detergent-resistant SM structures are arrow indicated. b Normalized (to 2908 cm⁻¹) hSRS spectra on the same NM (normal membrane, blue), SM (red), and LDs (green) structures in M381 cells from before (solid-lined) and after (dash-lined) detergent wash. (n = 7, 12, 6 for NM, SM, LD, respectively, blue arrow indicates the protein peak at 2940 cm⁻¹). c SRS images at the lipid (C-H) and the C-D channels on the same set of M381 cells growing in d₇-glucose medium with 1-day 5 μM CAY before (top) and after (bottom) detergent wash. d SRS images at the lipid (C-H) and the C-D channels on the same set of M381 cells with 3-day d₃₁-palmitic acid (d₃₁-PA) or d₃₅-stearic acid (d₃₅-ST) treatment before (top) and after (bottom) detergent wash. e Relative cellular viability with 3-day PA or ST treatment (n = 4 independent experiments). Scale bars, 20 μm. Data shown as mean ± SEM. Source data are provided as a Source data file.