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. 2020 Sep 24;11:4830. doi: 10.1038/s41467-020-18376-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Heatmap for the relative fatty acid abundances in designated classes of lipids from lipidomics of bulk M381 cells with (CAY) and without (CT) CAY (1 μM, 3 days) treatment. Each row represents a sample replicate and each column represents fatty acyl chains with increasing length and increasing double-bond numbers. SFA and UFA are categorized by pink and green, respectively. The abundance of FA of different chain length is normalized as a Z-score across all six samples within each column. TAG Triacylglycerol, DAG Diacylglycerol, CE Cholesteryl Ester, FFA Free Fatty Acids, PC Phosphatidylcholine, PE Phosphatidylethanolamine. b Overall concentration ratios between SFA to UFA (SFA/UFA), and between ST to OA (18:0/18:1) with CAY (purple) and without CAY (CT, pink, each value is shown as 100% reference) treatment from lipidomics of M381 cells in a (n = 3). c Concentration changes of 18:0 and 18:1 with CAY (purple) and without CAY (CT, pink) from lipidomics in a (n = 3 independent experiments). d Relative viability of M381 cells treated with 1 μM CAY with supplement of OA (18:1) at indicated concentration for 3 days (n = 4 independent experiments). e, Time-lapse apoptotic cell counts of M381 cells after treatment of 1 μM CAY with (pink) or without (purple) 10 μM OA (n = 3 independent experiments, data shown as mean ± error with 95% CI). f SRS imaging at the lipid channel before (left) and after (right) detergent wash on the same set of M381 cells treated with CAY and OA for 3 days. g Ranked pathways from analysis of gene expression trends on control (CT), CAY-treated (middle row, CAY) and CAY plus OA (CAY + OA) M381 cells. Scale bars, 20 μm. Data shown as mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001 from two-tailed unpaired t-tests. Lipidomics data are provided as Supplementary Data 1. Source data are provided as a Source data file.