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. 2020 Sep 24;10:15673. doi: 10.1038/s41598-020-72515-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology of cortical vesicles of zip9^+/+ and zip9^-/- oocytes throughout oogenesis. Representative images and average cortical vesicle diameters of (A) cortical alveoli, (B) early vitellogenic, (C) mid vitellogenic, and (D) late vitellogenic stage zip9^+/+ and zip9^-/- oocytes. Arrows indicate location of cortical vesicles. Scale bars: 12 µm. All data represents means ± SEM; n = 5 (average diameter of cortical vesicles for 5 females). Significance was determined by Welch's t-test (**, P < 0.01; ***, P < 0.001).