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. 2020 Sep 24;10:15673. doi: 10.1038/s41598-020-72515-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Zinc is stored in cortically-localized vesicles that undergo exocytosis upon egg activation. (A, B) zip9^+/+ (A) and zip9^-/- (B) eggs show intracellular zinc staining (arrows, 0 min) in cortically-localized vesicles that are reduced in number at 2.5–10 min post-activation. (C, D) Extracellular zinc is observed 2.5–10 min post-activation of zip9^+/+ (C) and zip9^-/- (D) eggs. (E) Time trace of normalized extracellular zinc fluorescence by ROI analysis (ROI denoted by yellow circle in C, 0 min) 0–10 min post-activation of zip9^+/+ and zip9^-/- eggs. Visualization was performed on eggs from a minimum of 3 females/strain. For (E), data represents means ± SEM; n = 5–6. Significance was determined by Welch's t-test (*, P < 0.05; **, P < 0.01).