Figure 1.
Sleeping Beauty Transposase Method to Model Glioma in Mice
(A) Generic plasmid maps used to generate gliomas in mice using the SB transposase method. GFP and Katushka are green and red fluorescent proteins, respectively. miR-30 sequences flank a shRNA encoding sequence designed to silence specific genes. The DNA transposons to be inserted are flanked by inverted repeats/direct repeats (IR/DR), which are recognized by the transposase.
(B) These sequences are then randomly integrated into the host genomic DNA sites, between bases T and A.
(C) Schematic of a 1 day-old mouse pup, showing the coordinates for plasmid injection into the lateral ventricle, at 1.5 mm rostral and 0.8 mm lateral to the lambda and 1.5 mm ventral.
(D) From left to right: bioluminescence scanning of a mouse pup 1 day after SB plasmid injection, when a large tumor has developed (106 photons/s/cm2/sr) and at tumor burden endpoint (107 photons/s/cm2/sr).