Figure 2.

Images Depicting Critical Steps of Tumor Dissection and CD45+ Cell Depletion Procedure

(A) Images showing a mouse SB-generated glioma under a stereo-zoom microscope equipped with a fluorescent light illumination system. In this example, the tumor was generated with SB plasmids encoding for genes coupled to GFP (middle picture) and Katushka (right picture) expression as reporter genes. Scale bars, 0.5 cm.

(B) Image showing a 50 mL conical tube, with a cell strainer on the top and a pestle, used to disintegrate the tumor tissue.

(C) Image illustrating MACS CD45-depletion column set up.