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. 2020 Jun 6;1(2):100044. doi: 10.1016/j.xpro.2020.100044

Table 1.

Plasmid Combinations to Generate Sleeping Beauty-Based Gliomas in Mouse

Plasmid Combination Addgene Cat. No. Genetic Alteration Name Median Survival (DPI) Reference
pT2/SB100x-Luc 20207 Luciferase expression NP 83–95 (Calinescu et al., 2015, Koschmann et al., 2016, Núñez et al., 2019, Comba et al., 2020)
pT/CAGGS-NRASV12 20205 NRASG12V overexpression
pT2/shP53 124261 p53 knock down
pT2/SB100x-Luc 20207 Luciferase expression NPA 69–80 (Koschmann et al., 2016, Núñez et al., 2019, Comba et al., 2020)
pT/CAGGS-NRASV12 20205 NRASG12V overexpression
pT2/shP53 124261 p53 knock down
pT2/shAtrx-GFP4 124259 Atrx knock down
pT2/SB100x-Luc 20207 Luciferase expression NPAI 163 (Núñez et al., 2019)
pT/CAGGS-NRASV12 20205 NRASG12V overexpression
pT2/shP53 124261 p53 knock down
pT2/shAtrx-GFP4 124259 Atrx knock down
pKT-IDH1(R132H)-Katushka 124257 IDH1R132H overexpression
pT2/SB100x-Luc 20207 Luciferase expression NPAH 162 (Garcia-Fabiani et al., 2020)
pT/CAGGS-NRASV12 20205 NRASG12V overexpression
pT2/shP53 124261 p53 knock down
pT2/shAtrx-GFP4 124259 Atrx knock down
pKT-H3.3(G34R)-Katushka - H3.3G34R overexpression
pT2/SB100x-Luc 20207 Luciferase expression NPAF 142 (Comba et al., 2020)
pT/CAGGS-NRASV12 20205 NRASG12V overexpression
pT2/shP53 124261 p53 knock down
pT2/shAtrx-GFP4 124259 Atrx knock down
pT2-shFYN-GFP4 - Fyn knock down
pT2/SB100x-Luc 20207 Luciferase expression NPF 131 (Comba et al., 2020)
pT/CAGGS-NRASV12 20205 NRASG12V overexpression
pT2/shP53 124261 p53 knock down
pT2-shFYN-GFP4 - Fyn knock down
pT2/SB100x-Luc 20207 Luciferase expression NPD 63–69 (Wiesner et al., 2009, Calinescu et al., 2015, Comba et al., 2020)
pT/CAGGS-NRASV12 20205 NRASG12V overexpression
pT2-shp53-PDGFβ-GFP4 - p53 knock down
PDGFβ overexpression
pT2/SB100x-Luc 20207 Luciferase expression NPDF 108 (Comba et al., 2020)
pT/CAGGS-NRASV12 20205 NRASG12V overexpression
pT2-shp53-PDGFβ-GFP4 - p53 knock down
PDGFβ overexpression
pT2-shFYN-GFP4 - Fyn knock down
pT2/SB100x-Luc 20207 Luciferase expression NL 30 (Wiesner et al., 2009, Calinescu et al., 2015)
pT/CMVSV40-LgT 20204 SV40-LgT overexpression
pT/CAGGS-NRASV12 20205 NRASG12V overexpression

As described in the text, one-day-old mouse pups are injected with a plasmid combination to introduce different genetic alterations into the sub-ventricular zone brain cells. The specific genetic background generated in these cells will define the aggressiveness of the tumor developed and the median survival of tumor bearing mice, measured as days post injection (DPI).