Reagent	Final Concentration	Amount
DMEM/F12	-	480 mL
Anti-Anti 100 X	1 X (1 IU/mL Penicillin, 100 μg/mL Streptomycin, 0.25 μg/mL Amphotericin B)	5 mL
N2 supplement 100 X	1 X	5 mL
B27 supplement 50 X	1 X	10 mL
Normocin	100 μg/mL	1 mL