Antibodies |
|
Phospho-Histone H3 (Ser10) - PE conjugated (Clone 11D8) |
Biolegend |
Catalog # 650807 |
|
Experimental Models: Organisms/Strains |
|
Mouse (C57BL/6J) |
Jackson Lab |
# 000664 |
|
Chemicals, Peptides, and Recombinant Proteins |
|
DMEM/F12 |
Thermo Fisher Scientific - Gibco |
Catalog # 11330032 |
DPBS, no calcium, no magnesium |
Thermo Fisher Scientific - Gibco |
Catalog # 14190144 |
BSA |
Millipore Sigma |
Catalog # A2153-100G |
N2 Supplement (100 X) |
Thermo Fisher Scientific - Gibco |
Catalog # 17502048 |
B27 Supplement (50 X) |
Thermo Fisher Scientific - Gibco |
Catalog # 17504044 |
Anti-Anti (100X) |
Corning |
Catalog # 30-004-CI |
Normocin |
InvivoGen |
Catalog # ant-nr-1 |
MACS BSA Stock Solution |
Miltenyi Biotec |
Catalog # 130-091-376 |
autoMACS Rinsing Solution |
Miltenyi Biotec |
Catalog # 130-091-222 |
mouse CD45 MicroBeads |
Miltenyi Biotec |
Catalog # 130-052-301 |
EGF |
PeproTech |
Catalog # AF-100-15 |
FGF |
PeproTech |
Catalog # 100-18B |
In vivo jetPEI (includes 10% glucose solution) |
Polyplus |
Catalog # 201-10G |
D-Luciferin Potassium Salt |
Goldbio |
Catalog # LUCK -3G |
Red Blood Cell (RBC) lysis buffer (10X) |
Biolegend |
Catalog # 420301 |
Fetal Bovine Serum (FBS) USDA Pure Premium |
Peak Serum |
Catalog # PS-FB3 |
|
Critical Commercial Assays |
|
Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit |
Thermo Fisher Scientific - Invitrogen |
Catalog # C10636
|
|
Recombinant DNA |
|
pT2/SB100x-Luc |
Addgene |
Catalog # 20207 |
pT/CAGGS-NRASV12 |
Addgene |
Catalog # 20205 |
pT2/shP53 |
Addgene |
Catalog # 124261 |
pKT-IDH1(R132H)-Katushka |
Addgene |
Catalog # 124257 |
|
Software and Algorithms |
|
FlowJo_V10 |
Becton Dickinson |
N/A |
|
Other |
|
LS Columns |
Miltenyi Biotec |
Catalog # 130-042-401 |
QuadroMACS Separator |
Miltenyi Biotec |
Catalog # 130-090-976 |
MACS MultiStand |
Miltenyi Biotec |
Catalog # 130-042-303 |
Pre-Separation Filters |
Miltenyi Biotec |
Catalog # 130-041-407 |
10 μl syringe |
Hamilton |
700 Series |
30 G hypodermic needle |
Hamilton |
Catalog # 7803-07. Specifications: 30GA RN 6PK 1.25" 15° |
Cell strainers |
Alkali Scientific Incorporated |
Catalog # MT4070 |
BD FACSAria™ III Flowcytometer |
Becton, Dickinson & Company |
N/A |
Just for Mouse Stereotaxic Instrument |
Stoelting |
Catalog # 51730 |
Quintessential Stereotaxic Injector (QSI) |
Stoelting |
Catalog # 53311 |
IVIS Spectrum In Vivo Imaging System |
Perkin Elmer |
Catalog # 124262 |
Allegra 6R Refrigerated Benchtop Centrifuge |
Beckman Coulter |
N/A |
Small Animal Heat Lamp |
Morganville Scientific |
Catalog # HL0100 |
Stereomicroscope System SZX7 (equipped with fluorescent lamp) |
Olympus |
N/A |
50 mL Olympus Polystyrene Tubes |
Genesee Scientific Corporation |
Catalog # 21-108 |
15 mL Olympus Polystyrene Tubes |
Genesee Scientific Corporation |
Catalog # 21-103 |
CellPro™ Flow Cytometry Tubes without cap, sterile, 12x75mm |
Alkali Scientific Incorporated |
Catalog # CT6414 |
Covidien Monoject 1 mL tuberculin Syringes |
Fisher Scientific |
Catalog # 22-257-154 |
Friedman Bone Rongeurs |
Stoelting |
Catalog # 52160P |
Micro Dissecting Scissors, 4.25 inch, Straight, 24mm, Sharp |
Stoelting |
Catalog # 52132-22P |
Brown-Adson Forceps, 4.7in, Side Teeth |
Stoelting |
Catalog # 52104-31P |
BD Precision Glide Hypodermic Needles 26G |
Fisher Scientific |
Catalog # 14-826-15 |