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. 2020 Jun 6;1(2):100044. doi: 10.1016/j.xpro.2020.100044
REAGENT or RESOURCE SOURCE IDENTIFIER
Antibodies

Phospho-Histone H3 (Ser10) - PE conjugated (Clone 11D8) Biolegend Catalog # 650807

Experimental Models: Organisms/Strains

Mouse (C57BL/6J) Jackson Lab # 000664

Chemicals, Peptides, and Recombinant Proteins

DMEM/F12 Thermo Fisher Scientific - Gibco Catalog # 11330032
DPBS, no calcium, no magnesium Thermo Fisher Scientific - Gibco Catalog # 14190144
BSA Millipore Sigma Catalog # A2153-100G
N2 Supplement (100 X) Thermo Fisher Scientific - Gibco Catalog # 17502048
B27 Supplement (50 X) Thermo Fisher Scientific - Gibco Catalog # 17504044
Anti-Anti (100X) Corning Catalog # 30-004-CI
Normocin InvivoGen Catalog # ant-nr-1
MACS BSA Stock Solution Miltenyi Biotec Catalog # 130-091-376
autoMACS Rinsing Solution Miltenyi Biotec Catalog # 130-091-222
mouse CD45 MicroBeads Miltenyi Biotec Catalog # 130-052-301
EGF PeproTech Catalog # AF-100-15
FGF PeproTech Catalog # 100-18B
In vivo jetPEI (includes 10% glucose solution) Polyplus Catalog # 201-10G
D-Luciferin Potassium Salt Goldbio Catalog # LUCK -3G
Red Blood Cell (RBC) lysis buffer (10X) Biolegend Catalog # 420301
Fetal Bovine Serum (FBS) USDA Pure Premium Peak Serum Catalog # PS-FB3

Critical Commercial Assays

Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit Thermo Fisher Scientific - Invitrogen Catalog # C10636

Recombinant DNA

pT2/SB100x-Luc Addgene Catalog # 20207
pT/CAGGS-NRASV12 Addgene Catalog # 20205
pT2/shP53 Addgene Catalog # 124261
pKT-IDH1(R132H)-Katushka Addgene Catalog # 124257

Software and Algorithms

FlowJo_V10 Becton Dickinson N/A

Other

LS Columns Miltenyi Biotec Catalog # 130-042-401
QuadroMACS Separator Miltenyi Biotec Catalog # 130-090-976
MACS MultiStand Miltenyi Biotec Catalog # 130-042-303
Pre-Separation Filters Miltenyi Biotec Catalog # 130-041-407
10 μl syringe Hamilton 700 Series
30 G hypodermic needle Hamilton Catalog # 7803-07. Specifications: 30GA RN 6PK 1.25" 15°
Cell strainers Alkali Scientific Incorporated Catalog # MT4070
BD FACSAria™ III Flowcytometer Becton, Dickinson & Company N/A
Just for Mouse Stereotaxic Instrument Stoelting Catalog # 51730
Quintessential Stereotaxic Injector (QSI) Stoelting Catalog # 53311
IVIS Spectrum In Vivo Imaging System Perkin Elmer Catalog # 124262
Allegra 6R Refrigerated Benchtop Centrifuge Beckman Coulter N/A
Small Animal Heat Lamp Morganville Scientific Catalog # HL0100
Stereomicroscope System SZX7 (equipped with fluorescent lamp) Olympus N/A
50 mL Olympus Polystyrene Tubes Genesee Scientific Corporation Catalog # 21-108
15 mL Olympus Polystyrene Tubes Genesee Scientific Corporation Catalog # 21-103
CellPro™ Flow Cytometry Tubes without cap, sterile, 12x75mm Alkali Scientific Incorporated Catalog # CT6414
Covidien Monoject 1 mL tuberculin Syringes Fisher Scientific Catalog # 22-257-154
Friedman Bone Rongeurs Stoelting Catalog # 52160P
Micro Dissecting Scissors, 4.25 inch, Straight, 24mm, Sharp Stoelting Catalog # 52132-22P
Brown-Adson Forceps, 4.7in, Side Teeth Stoelting Catalog # 52104-31P
BD Precision Glide Hypodermic Needles 26G Fisher Scientific Catalog # 14-826-15