Table 1. Mice and cells used.
Experiment | Samples used |
---|---|
TEM | 3-week-old male wild-type CD1 mice from Charles River |
quantification of centrioles per OSN and centrioles per NeuroD1-positive progenitor | adult (1 to 14 months) TgCAG-Arl13b-mCherry, eGFP-centrin2 mice from Jackson Laboratories [17] |
immunofluorescent staining in olfactory epithelium | adult (7 to 12 months) C57BL/6 mice and TgCAG-Arl13b-mCherry, eGFP-centrin2 mice from Jackson Laboratories [17] embryonic stage E12.5 TgCAG-Arl13b-mCherry, eGFP-centrin2 mice from Jackson Laboratories [17] |
centriole area analysis in cell culture | hTert RPE-1 TetOn-Plk4-Flag, eGFP-centrin2 cells (a gift from Bryan Tsou) |
Arl13b-mCherry, ADP-ribosylation-factor-like GTPase 13b; eGFP, enhanced green fluorescent protein; NeuroD1, neuronal differentiation 1; OSN, olfactory sensory neuron; RPE-1, retinal pigmented epithelial; TEM, transmission electron microscopy.