Table 2. Primary antibodies used.
| Target | Source | Dilution | Treatment |
|---|---|---|---|
| β tubulin III | BioLegend, clone TuJ1 | 1:2,000 | none needed |
| γ tubulin | Sigma-Adrich, clone GTU88 | 1:1,000 | none needed |
| centrin (all isotypes) |
EMD Millipore, clone 20H5 | 1:5,000 | 10-minute pretreatment in 0.5% (w/v) sodium dodecyl sulfate in PBS for olfactory epithelium |
| phospho-H3, Ser10 | Cell Signaling, #53348T | 1:200 | none needed |
| GFP | Invitrogen, #A-11120 | 1:1,000 | none needed |
| Sass6 | Santa Cruz Biotech, #91.390.21 | 1 μg/mL | requires cell fixation in −20°C methanol |
| NeuroD1 | Proteintech, #12081-1-AP | 1:100 | 10-minute pretreatment in 0.5% (w/v) sodium dodecyl sulfate in PBS, requires overnight incubation in primary antibody |
NeuroD1, neuronal differentiation 1, w/v, weight in grams per 100 mL volume.