Figure 1. Heteroarylation of the reactive Lys residue of catalytic antibody h38C2.
(A) Proposed reaction of MS-PODA with the ε-amino group of Lys99 under mild conditions. The side chains of the flanking Cys and threonine (Thr) residues are shown as R1 and R2, respectively. (B) In silico docking model of PODA-conjugated Lys99 in the hydrophobic pocket (box) of h38C2. The Fab’s heavy chain (VH-CH1) is shown in blue and the light chain (VL-CL) in green. Interatomic distances given in Å were calculated by PyMOL software.