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. Author manuscript; available in PMC: 2021 Feb 15.
Published in final edited form as: Cancer Res. 2020 Jun 10;80(16):3305–3318. doi: 10.1158/0008-5472.CAN-20-0057

Fig. 5. PPP2R2A depletion-induced replication stress is associated with increased c-Myc expression.

Fig. 5.

(A) PPP2R2A downregulation by two different shRNAs leads to increased replication stress and c-Myc in H1299 and A549 cells. (B) Stable overexpression of PPP2R2A in H1437 cells decreased the expression of c-Myc and γ-H2AX. (C) Statistical analysis of the protein expression levels of c-Myc relative to β-actin in H1299 and A549 cells with or without PPP2R2A knockdown. Data are presented as the mean ± SEM (n=3). (D) Statistical analysis of mRNA levels of c-Myc in H1299 and A549 cells with or without PPP2R2A knockdown. Data were normalized by the amount of GAPDH mRNA, data are the mean ± SEM (n=3). The statistical significance of C and D was determined by one-way ANOVA followed by Bonferroni’s post hoc analysis for multiple comparisons. *P < 0.05 and ****P < 0.0001. (E) PPP2R2A knockdown leads to upregulation of c-Myc in the PPP2R5A-depleted cells by shRNA.