Figure 1.

Two validated splice variants ZHX2 and MKNK2 ASEs.

Alternative start site in ZHX2 (A.) and the deletion event in MKNK2 (B.) Sashimi plots of representative controls (N = Normal, light) and representative tumours (T = Tumour, dark) are shown along with the designed WT and ASE primer-probe assays. Sashimi plots display downsampled RPM counts using IGV viewer of given junction transcripts. Boxplots show the results of ASE qRT-PCR validation in n = 22 UPPP non-cancer controls and n = 31 HPV+ OPSCC samples. Mann–Whitney U test revealed significant overexpression of ASE (normalized to WT expression) within tumours for both splice variants (p-values <0.001).