Figure 5.

Hypoxia leads to aberrant expression of ANGPTL4 and HIF-1α, positively correlated with their aberrant hydroxymethylation levels in promoter regions. (a, b) Western blot results showed the protein levels of HIF-1α and ANGPTL4 under normoxic conditions with 21%O₂ and under hypoxic conditions with 10% and 1% O₂ for 24 hours and 48 hours. Expression of HIF-1α and ANGPTL4 both highly expressed in 10%O₂-48 hours and reduced expressed in 1%O₂-48 hours. (c, d) The 5hmC levels in the promoters of HIF-1α and ANGPTL4 under normoxic and hypoxic conditions were detected by hMeDIP-qPCR. The percentage of the input is shown as indicated. The results showed that the 5hmC enrichment of HIF-1α and ANGPTL4 increased in 10%O₂-48 hours and reduced in 1%O₂-48 hours. (*p < 0.05, **p < 0.01).