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. 2020 Sep 11;35(10):2294–2302. doi: 10.1093/humrep/deaa199

Table II.

Effect of Ala515Val mutation on AMH production.

hAMH-RAQR-515Ala hAMH-RAQR-515Val hAMH-RARR-515Ala hAMH-RARR-515Val
Cell lysate* (ng/ml) 62.68 Non-detected 1027.12 Non-detected
Supernatant* (ng/ml) 8486.5 83.08 91361.95 122.41
Supernatant# (ng/ml) 94.6 181.7 2431.7 182.8
*

AMH was measured in cell lysates and supernatants of HEK293 cells stably transfected with hAMH-515Ala or hAMH-515Val expression constructs without (RAQR) or with (RARR) the optimized cleavage site using the picoAMH assay (Ansh Labs), indicated by*, or the automated Lumipulse G1200 (Fujirebio), indicated by #.