TABLE 3.

List of some web-based tools to design CRISPR/Cas systems.

Tool	Features	Web address	Reference
CRISPR Design	sgRNA designing, prediction of off-site targeting against a limited number of reference genomes including Arabidopsis thaliana,	http://crispr.mit.edu	70
Cas-OFFinder	Multi-featured, prediction of potential off-site targeting against many reference genomes, sgRNA designing, PAM type option for different CRISPR/Cas variants	http://www.rgenome.net/cas-offinder/	71
CCTop	sgRNA target selection, off-target prediction	http://crispr.cos.uni-heidelberg.de/	72
CHOPCHOP	Multi-featured, Target sites for CRISPR/TALENs systems, Off-site targeting against many reference genomes	http://chopchop.cbu.uib.no	73
E-CRISP	Multi-featured, Designing of CRISPR constructs, Prediction of off-site targeting against a limited number of genomes	http://www.e-crisp.org/E-CRISP/reannotate_crispr.html	74
CROP-IT	CRISPR/Cas9 target sites in the input sequence, prediction of off-site targeting	http://www.adlilab.org/CROP-IT/homepage.html	75
CRISPR-P	sgRNA designing in plants, prediction of off-site targeting	http://cbi.hzau.edu.cn/crispr/	76
CRISPRseek	Target-specific sgRNA design tool, Prediction of off-site targeting	http://bioconductor.org/packages/release/bioc/html/CRISPRseek.html	77
CRISPR-PLANT	CRISPR/Cas9-sgRNAs designing tool limited to plants	http://genome.arizona.edu/crispr/	78
SYNTHEGO-tool	Multi-featured, Design and validate CRISPR sgRNAs, Prediction of off-site targeting	https://design.synthego.com/#/