Figure 1.
Expression of SlEAD1 in response to ABA treatment. The expression of SlEAD1 was down-regulated by ABA treatment. Fourteen-day-old Micro-Tom wild type seedlings were cut into pieces and treated in darkness for 4 h with 50 µM ABA or solvent methanol as a control. Samples were frozen in liquid N2, and total RNA was isolated and used for cDNA synthesis. The synthesized cDNA was used as template for qRT-PCR to examine the expression of SlEAD1, and the expression of SlACT2 was examined and used as an inner control. The expression level of SlEAD1 in mock-treated samples was set as 1, and its relative expression level in ABA treated samples was calculated. Data represent the mean ± SD of three replicates. *Significantly different from that in the mock-treated samples (p < .001).