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Intracellular granulysin expression analysis in human PBMC after 48 and 72 h of treatment with D. oil at 10 μg/ml. Untreated PBMC (control) and Con A treated PBMC were taken as negative and positive controls, respectively. Treated PBMC were fixed, permeabilized and probed with anti-granulysin antibody. Followed by treatment with secondary antibody (anti-rabbit IgG Allophycocyanin) and flow cytometry analysis. Results were expressed as percentage of granulysin positive cells
