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. 2020 Sep 24;10:15711. doi: 10.1038/s41598-020-72594-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Role of P-STAT3^S727 in accumulation of LC3 in mitochondria of H. pylori infected AGS cells. (A) Immunocytochemical analysis of LC3 co-localized with mitochondria. AGS cells were treated with vehicle (DMSO) or CCCP (1 μM) for 1 h. MitoTracker was used as a selective probe of mitochondria. Scale bar, 100 μm. (B) Western blot analysis of P-STAT3^Ser727, STAT3, and LC3B in AGS cells treated with DMSO or 1 μM CCCP for 1 h. (C) AGS cells were treated with DMSO or CCCP (1 μM) for 1 h. Western blot analysis of P-STAT3^Ser727 and LC3B in cytosolic and mitochondrial extracts. VDAC and α-tubulin are mitochondrial and cytoplasmic protein markers, respectively. (D) Western blot analysis of P-STAT3^Ser727 and LC3B in AGS cells transfected with WT or serine dominant negative mutant vector. The relative expression levels of LC3 from three independent experiments are presented means ± S.D. (E) Immunocytochemical analysis of LC3B in WT and Ser727 mutant AGS cells with or without H. pylori infection. MitoTracker is a selective probe of mitochondria. Scale bar, 100 μm.