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. 2020 Sep 24;10:15686. doi: 10.1038/s41598-020-72610-6

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cellular clustering of YFV epitope-specific CD4⁺ T cells pre and post YF-Vax vaccination. (a) UMAP and PhenoGraph analysis of surface marker expression of YFV ENV-specific, YFV NS1-specific, YFV NS3- specific CD4⁺ T cells for all 9 subjects at all time points (n = 58). A total of 5246 YFV ENV-specific, 8005 YFV NS1- specific and 4600 YFV NS3-specific cells as identified by YFV-specific tetramer staining were included in the analysis. PhenoGraph defined a total of 11 different clusters. (b) Heatmap of the surface marker expression level of cells within these 11 clusters with percentage of cells that were positive for each marker. Markers that are used to define T cell subsets or activation state are boxed. (c) Distribution of YFV ENV-specific, YFV NS1-specific and YFV NS3-specific CD4⁺ T cells in UMAP.