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. 2020 Sep 24;10:15613. doi: 10.1038/s41598-020-72391-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Changes with time in phosphorylation profile upon overexpression of Ppz1. (a) The significance versus fold-change in phosphorylation (y and x axes, respectively) of the 6,008 unique phosphosites identified in this work and their variation along time with overexpression of Ppz1 is represented as a Volcano plot. Fold-change is calculated as the ratio of Ppz1-overexpressing cells vs control cells, for each time and compared with t = 0. Shadows denote regions in which log(2) value of the change is ≥ 1 (phosphorylated) or ≤ − 1 (dephosphorylated), and p-value ≤ 0.05. (b) Data for a total number of 230 dephosphorylated (right panel) and 85 phosphorylated phosphopeptides (left panel) showing statistically significant changes at least at one time-point was log(2) transformed and plotted. The discontinuous line marks the zero point in the X-axis. (c) Variation of the number of phospho- (filled circle) and dephosphorylated (open circle) proteins along the experiment.