Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 25;10:15765. doi: 10.1038/s41598-020-71550-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Catalytic immuno-subunit β5i plays an important role in the degradation of oxidized proteins by β5i-containing 20S proteasomes. (a) Western blot analysis of the kinetics of degradation of oxidized calmodulin by the four proteasome subtypes in the presence or absence of the β5i-specific inhibitor PR-957 (1 μM). These results are representative of three independent experiments. (b) Assessing the degradation of the radiolabeled oxidized hemoglobin by the four proteasome subtypes in the presence or in the absence of the β5i-specific inhibitor PR-957 after 24 h of digestion. The percentage of degraded hemoglobin was reported in each case to the percentage of degraded hemoglobin by the SP in the absence of PR-957, and the absolute percentages of degraded hemoglobin in the vehicle condition are indicated on the histogram. Error bars are SEM of three independent experiments. (c) Western blot analysis of the kinetics of degradation of oxidized calmodulin by the intermediate proteasome β5i treated with increased concentrations of the PR-957 inhibitor. These results are representative of three independent experiments. Full-length images for (a) and (c) are presented in Fig. S13.