Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Sep 25;10:15808. doi: 10.1038/s41598-020-72875-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Endogenous Myh11+ mural cells on the retinal microvasculature exhibit a myofibroblast phenotype after a chemical burn to the murine sclera. (A) Model demonstrating that silver-nitrate-burn injury to the sclera induced formation of retinal fibrotic scar tissue. (B) Immunostained, uninjured retinal tissue revealed Myh11+ mural cells, labeled by tdTomato, are found only on the CD31+ retinal microvasculature. Col-IV is expressed only in the basement membrane of the retinal microvasculature. Scale bar, 15 µm. (C) Immunostained retinas one-month post-burn injury showed multiple off-vessel Myh11+ mural cells (tdTomato+), which is indicated by the lack of overlap with the blood vessel endothelium marker CD31. (D,E) Off-vessel Myh11+ mural cells display αSMA+ stress fibers and are positive for Col-IV, Col-III, and F-actin as shown by fluorescently labeled phalloidin. Scale bar, 15 µm. Animals were tested beginning at 10–12 weeks of age. Immunohistochemistry images are representative of three uninjured and injured eyes of Myh11-tdTomato mice. Field of view in injured eyes were selected based on the visual observation of off-vessel Myh11+ mural cells.