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. 2020 Sep 25;10:15808. doi: 10.1038/s41598-020-72875-x

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Intravitreally injected Myh11-derived MSCs accelerate microvasculature recovery and adopt a perivascular position during murine oxygen-induced retinopathy (OIR). (A) Diagram illustrating the timeline of the murine OIR model and intravitreal injection of Myh11-derived MSCs. After hyperoxia injury from P7 to P12, pup eyes were intravitreally injected with PBS-vehicle or 10,000 Myh11-derived MSCs at passage 3–5, and analyzed at P14 and P17 post-injection. (B) At P14 and P17, intravitreally injected Myh11-derived MSCs (DiI+/eYFP+) were able to integrate into the retinal tissue and associate with Col-IV+ retinal vasculature. Scale bar, 10 µm. (C) Representative immunostained retinal flatmounts at P14 and P17 are shown with outlined area (yellow) representing capillary dropout region caused by OIR. Retinal blood vessels were immunostained with lectin (red). Quantification of capillary dropout in ImageJ showed eyes intravitreally injected with Myh11-derived MSCs experienced a significant reduction in capillary dropout area at P14 (n = 7 paired eyes), and at P17 (n = 10 paired eyes) when compared to the contralateral PBS vehicle control eyes. Scale bar, 1000 µm. *(p < 0.05). All data were analyzed using a Wilcoxon test.