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. 2020 Sep 24;10:15623. doi: 10.1038/s41598-020-72492-8

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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(a) VOI location in a female mouse brain at P40 using the fsems images used for positioning. (b) Typical in vivo ³¹P MR spectra of the mouse brain from P20 to P250 at 14.1 T (black, no baseline correction, 10 Hz line broadening). The total spectral fit (gray) determined by the LCModel, the baseline, the individual fits of α-ATP, NADH (red), NAD⁺ (blue), and the fitting residual. (c) Zoom on the summed spectral region of NAD⁺ and NADH with the following number of animals at a given postnatal days: P20, N = 10; P40, N = 8; P90, N = 8; P250, N = 5. The figure clearly depicts the changes with development in the NAD concentrations.