Table 2.
Inhibition halo (IH) diameter, minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) obtained on treating microorganisms with the essential oils from leaves and flowers of S. hydrangea.
Microorganism | Leaves | Flowers | Antibiotics | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Rifampin | Gentamicin | Nystatin | ||||||||||
IH (mm) | MIC (µg/mL) | MBC (µg/mL) | IH (mm) | MIC (µg/mL) | MBC (µg/mL) | IH (mm) | MIC (µg/mL) | IH (mm) | MIC (µg/mL) | IH (mm) | MIC (µg/mL) | |
Shigella dysenteriae | ND | 62.5 | 62.5 | ND | 62.5 | 125 | 9 ± 0.00b | 15.63 | 17 ± 0.01a | 3.90 | NA | NA |
Pseudomonas aeruginosa | ND | 15.63 > | 15.63 > | ND | 15.63 > | 15.63 > | ND | 31.25 | 20 ± 0.00a | 7.81 | NA | NA |
Bacillus subtilis | 9.50 ± 0.00 c | 250 | > 1000 | ND | 31.25 | 31.25 | 19 ± 0.00b | 31.25 | 30 ± 0.02a | 3.90 | NA | NA |
Staphylococcus epidermidis | 9.50 ± 0.00d | 1000 | 1000 | 10.67 ± 1.15c | 250 | 250 | 44 ± 0.01a | 1.95 | 39 ± 0.00b | 1.95 | NA | NA |
Escherichia coli | ND | 1000 | 1000 | ND | 31.25 | 125 | 10 ± 0.00b | 15.63 | 23 ± 0.02a | 31.25 | NA | NA |
Staphylococcus aureus | 9.50 ± 0.50 c | 500 | > 1000 | ND | 125 | 125 | 21 ± 0.01b | 31.25 | 27 ± 0.03a | 1.95 | NA | NA |
Klebsiella pneumoniae | ND | 62.50 | 62.5 | ND | 62.5 | 62.5 | 8 ± 0.00b | 15.63 | 17 ± 0.03a | 3.90 | NA | NA |
Proteus vulgaris | ND | 250 | 250 | ND | 125 | 125 | 8 ± 0.00b | 15.63 | 24 ± 0.04a | 15.63 | NA | NA |
Salmonella paratyphi-A | ND | 125 | 125 | ND | 62.5 | 125 | 8 ± 0.01b | 15.63 | 18 ± 0.01a | 3.90 | NA | NA |
Candida albicans | ND | 250 | 250 | ND | 1000 | 1000 | NA | NA | NA | NA | 33 ± 0.01a | 125 |
Aspergillu sniger | ND | > 2000 | > 2000 | ND | 2000 | 2000 | NA | NA | NA | NA | 27 ± 0.00a | 31.2 |
Aspergillus brasiliensis | ND | > 2000 | > 2000 | ND | 2000 | 2000 | NA | NA | NA | NA | 30 ± 0.01a | 31.2 |
NA indicates no activity and ND indicates not determined. Mean values ± standard deviations of three cultures were reported (n = 3).
Values with different letters are statistically different (Duncan, p ≤ 0.05).