Table 2.
Antibodies used for western blotting.
| Primary antibodies | Secondary antibodies | ||||
|---|---|---|---|---|---|
| Antibody | Catalogue number/distributed by | Dilution | Catalogue number/distributed by | Dilution | Specificity |
| NIS | NBP1-70342/Novus Biological Centennial, CO, USA | 1:1000 | B8520/Sigma-Aldrich MO, USA | 1:10,000 | Anti-mouse |
| TPO | sc-58432/Santa Cruz Biotechnology MA, USA | 1:1000 | B8520/Sigma-Aldrich MO, USA | 1:10,000 | Anti-mouse |
| MCT8 | sc-47124/Santa Cruz Biotechnology MA, USA | 1:500 | AP106B/Millipore Corporation CA, USA | 1:10,000 | Anti-goat |
| TSHr | sc-53542/Santa Cruz Biotechnology MA, USA | 1:500 | B8520/Sigma-Aldrich MO, USA | 1:10,000 | Anti-mouse |
| Cyclophilin | #51418/Cell Signaling Technology MA, USA | 1:1000 | B7389/Sigma-Aldrich MO, USA | 1:10,000 | Anti-rabbit |
| SOD | sc-133134/Santa Cruz Biotechnology MA, USA | 1:500 | B8520/Sigma-Aldrich MO, USA | 1:10,000 | Anti-mouse |
| CAT | sc-50508/Santa Cruz Biotechnology MA, USA | 1:1000 | B7389/Sigma-Aldrich MO, USA | 1:10,000 | Anti-rabbit |
| GPx | sc-133152/Santa Cruz Biotechnology MA, USA | 1:1000 | B8520/Sigma-Aldrich MO, USA | 1:10,000 | Anti-mouse |
| 4-HNE | ab46545/Abcam, MA, USA | 1:1000 | B7389/Sigma-Aldrich MO, USA | 1:10,000 | Anti-rabbit |
| AR | 06-680/Millipore Corporation, Temecula, CA, USA | 1:1000 | B7389/Sigma-Aldrich MO, USA | 1:10,000 | Anti-rabbit |
| ERα | 06-935/Millipore Corporation, Temecula, CA, USA | 1:500 | B8520/Sigma-Aldrich MO, USA | 1:10,000 | Anti-mouse |
| GAPDH | 14C10 #2118/Cell Signaling Technology MA, USA | 1:1000 | B7389/Sigma-Aldrich MO, USA | 1:10,000 | Anti-rabbit |
NIS sodium/iodide symporter, TPO thyroid peroxidase, MCT8 monocarboxylate transporter-8, TSHr thyroid-stimulating hormone receptor, SOD superoxide dismutase, CAT catalase, GPx glutathione peroxidase, 4-HNE 4-hydroxynonenal, AR androgenic receptor, ERα estrogen receptor alpha, GAPDH glyceraldehyde 3 phosphate dehydrogenase.