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. 2020 Sep 25;11:4855. doi: 10.1038/s41467-020-18634-y

Fig. 2. ACKR3 stimulation inhibits intercellular dye coupling in astrocytes.

Fig. 2

Representative photomicrographs of scrape loading performed in confluent primary astrocyte cultures, taken ten minutes after the scrape in the presence of Lucifer yellow (1 mg/mL). All treatments (CXCL12 (10 nM), AMD3100 (1 μM), CXCL11 (100 nM), NBI-74330 (1 μM)) were applied for 30 min except for CBX (50 μM) and PTX (100 ng/mL) that were applied overnight. Scale bar = 200 μm. LY diffusion was calculated by measuring the distance from the scrape where LY fluorescence intensity is 50% of the maximal fluorescence. Values were normalized to LY diffusion in vehicle-treated astrocytes. Results represent the means ± SEM. One-way ANOVA was used for comparison within the vehicle-treated group F(11,24), whereas the two-way ANOVA was used for the comparison across vehicle and PTX groups F(3,16). For both Bonferroni post-hoc was used. See the Statistics and Reproducibility section for the number of repetitions, exact P values and symbol (*,$) legend. Source data are provided as a Source Data file.